Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution

Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution

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Stochastic and proliferative events initiated from a single cell can disrupt homeostatic balance and lead to fatal disease processes such as Soccer - Clothing Womens Bottoms - Shorts cancer metastasis.To overcome metastasis, it is necessary to detect and quantify sparsely distributed metastatic cells throughout the body at early stages.Here, we demonstrate that clear, unobstructed brain/body imaging cocktails and computational analysis (CUBIC)-based cancer (CUBIC-cancer) analysis with a refractive index (RI)-optimized protocol enables comprehensive cancer cell profiling of the whole body and organs.We applied CUBIC-cancer analysis to 13 mouse models using nine cancer cell lines and Highchair Decorations spatiotemporal quantification of metastatic cancer progression at single-cell resolution.CUBIC-cancer analysis suggests that the epithelial-mesenchymal transition promotes not only extravasation but also cell survival at metastatic sites.

CUBIC-cancer analysis is also applicable to pharmacotherapeutic profiling of anti-tumor drugs.CUBIC-cancer analysis is compatible with in vivo bioluminescence imaging and 2D histology.We suggest that a scalable analytical pipeline with these three modalities may contribute to addressing currently incurable metastatic diseases.